Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy

Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy Full article

Journal

Angewandte Chemie - International Edition
ISSN: 1433-7851 , E-ISSN: 1521-3773

Output data

Year: 2019, Volume: 58, Number: 38, Pages: 13271-13275 Pages count : 5 DOI: 10.1002/anie.201904152

Tags

distance measurements; EPR spectroscopy; fullerenes; PELDOR; spin labels

Authors

Krumkacheva O.A. ^1,4 , Timofeev I.O. ^1,4 , Politanskaya L.V. ^2,4 , Polienko Y.F. ^2,4 , Tretyakov E.V. ^2,4 , Rogozhnikova O.Y. ^2,4 , Trukhin D.V. ^2,4 , Tormyshev V.M. ^2,4 , Chubarov A.S. ^3,4 , Bagryanskaya E.G. ^2,4 , Fedin M.V. ^1,4

Affiliations

1	(Scopus) International Tomography Center SB RAS, Novosibirsk, 630090, Russian Federation
2	(Scopus) N.N.Vorozhtsov Institute of Organic Chemistry SB RAS, Novosibirsk, 630090, Russian Federation
3	(Scopus) Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090, Russian Federation
4	(Scopus) Novosibirsk State University, Novosibirsk, 630090, Russian Federation

Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties of the spin labels used in this approach determine the sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose and validate the use of photoexcited fullerenes as spin labels for pulsed dipolar (PD) EPR distance measurements. Hyperpolarization and the narrower spectrum of fullerenes compared to other triplets (e.g., porphyrins) boost the sensitivity, and superior relaxation properties allow PD EPR measurements up to a near-room temperature. This approach is demonstrated using fullerene–nitroxide and fullerene–triarylmethyl pairs, as well as a supramolecular complex of fullerene with nitroxide-labeled protein. Photoexcited triplet fullerenes can be considered as new spin labels with outstanding spectroscopic properties for future structural studies of biomolecules. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Krumkacheva O.A. , Timofeev I.O. , Politanskaya L.V. , Polienko Y.F. , Tretyakov E.V. , Rogozhnikova O.Y. , Trukhin D.V. , Tormyshev V.M. , Chubarov A.S. , Bagryanskaya E.G. , Fedin M.V.
Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy
Angewandte Chemie - International Edition. 2019. V.58. N38. P.13271-13275. DOI: 10.1002/anie.201904152 WOS Scopus РИНЦ OpenAlex

Published print:

Sep 16, 2019

Web of science:	WOS:000480487000001
Scopus:	2-s2.0-85070710781
Elibrary:	41628372
OpenAlex:	W2963929366

DB	Citing
Scopus	34
Web of science	25
OpenAlex	42