Lipophilic Metabolites from Five-Needle Pines, Pinus armandii and Pinus kwangtungensis, Exhibiting Antibacterial Activity

Lipophilic Metabolites from Five-Needle Pines, Pinus armandii and Pinus kwangtungensis, Exhibiting Antibacterial Activity Full article

Journal

Chemistry and Biodiversity
ISSN: 1612-1872 , E-ISSN: 1612-1880

Output data

Year: 2020, Volume: 17, Number: 8, Article number : e2000201, Pages count : DOI: 10.1002/cbdv.202000201

Tags

antibacterial activity; Bacillus subtilis; GC/MS; Pinus armandii; Pinus kwangtungensis; Serratia marcescens

Authors

Shpatov A.V. ¹ , Frolova T.S. ^2,3 , Popov S.A. ¹ , Sinitsyna O.I. ^2,3 , Salnikova O.I. ¹ , Zheng G. ⁴ , Yan L. ⁴ , Sinelnikova N.V. ⁵ , Pshennikova L.M. ⁶ , Kochetov A.V. ²

Affiliations

1	(Scopus) Medicinal Chemistry Laboratory, Novosibirsk Institute of Organic Chemistry (NIOC), Acad. Lavrentyev Ave. 9, Novosibirsk, 630090, Russian Federation
2	(Scopus) Laboratory of Genetic Engineering, Institute of Cytology and Genetics (ICG), Acad. Lavrentyev Ave. 10, Novosibirsk, 630090, Russian Federation
3	(Scopus) Department of Natural Sciences, Novosibirsk State University (NSU), Pirogov Str. 2, Novosibirsk, 630090, Russian Federation
4	(Scopus) National Engineering Laboratory for Biomass Chemical Utilization, Institute of Chemical Industry of Forest Products (ICIFP), Chinese Academy of Forestry, 16 Suojin Wucun, Nanjing, 210042, China
5	(Scopus) Laboratory of Botany, Institute of Biological Problems of the North (IBPN), Portovaya Str. 18, Magadan, 685000, Russian Federation
6	(Scopus) Laboratory of Introduction and Selection, Botanical Garde?Institute (BGI), Makovskiy Str. 142, Vladivostok, 690024, Russian Federation

Lipophilic extractive metabolites from needles and defoliated twigs of Pinus armandii and P. kwangtungensis were studied by GC/MS. Needles of P. armandii contained predominantly 15-O-functionalized labdane type acids (anticopalic acid), fatty acids, nonacosan-10-ol, sterols, nonacosan-10-ol and sterol saponifiable esters, and acylglycerols, while P. kwangtungensis needles contained no anticopalic acid, but more trinorlabdane (14,15,16-trinor-8(17)-labdene-13,19-dioic acid) and other labdane type acids, nonacosan-10-ol and its saponifiable esters. The major compounds in the P. armandii defoliated twig extract were abietane and isopimarane type acids, fatty acids, sterols, labdanoids (cis-abienol), cembranoids (isocembrol and 4-epi-isocembrol), saponifiable sterol esters, and acylglycerols. The same extract of P. kwangtungensis contained larger quantities of fatty acids, caryophyllene oxide, serratanoids, sterols, saponifiable sterol esters, and acylglycerols, but lesser amounts of abietane and isopimarane type acids, cis-abienol, and lacked cembranoids. Both twig and needle extracts of P. armandii and P. kwangtungensis, as well as the extracts’ fractions, significantly inhibited the growth of Gram-negative bacteria Serratia marcescens with MIC of 0.1 mg ml−1, while in most cases they slightly stimulated the growth of Gram-positive bacteria Bacillus subtilis at the same concentrations. Thus, lipophilic extractive compounds from the needles and defoliated twigs of both pines are prospective for the development of antiseptics against Gram-negative bacteria. © 2020 Wiley-VHCA AG, Zurich, Switzerland

Shpatov A.V. , Frolova T.S. , Popov S.A. , Sinitsyna O.I. , Salnikova O.I. , Zheng G. , Yan L. , Sinelnikova N.V. , Pshennikova L.M. , Kochetov A.V.
Lipophilic Metabolites from Five-Needle Pines, Pinus armandii and Pinus kwangtungensis, Exhibiting Antibacterial Activity
Chemistry and Biodiversity. 2020. V.17. N8. e2000201 . DOI: 10.1002/cbdv.202000201 WOS Scopus OpenAlex

Published online:	Jul 3, 2020
Published print:	Aug 1, 2020

Web of science:	WOS:000544979100001
Scopus:	2-s2.0-85087508083
OpenAlex:	W3025719259

DB	Citing
Scopus	6
Web of science	5
OpenAlex	6