A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level Full article
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Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620 |
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| Output data | Year: 2017, Volume: 43, Number: 2, Pages: 211-215 Pages count : 5 DOI: 10.1134/S1068162017020121 | ||||||||
| Tags | juvenile hormone; tritium; JH esterase; Drosophila; Galleria | ||||||||
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Abstract:
A method of insect juvenile hormone III (JH-III) labeling by thermally activated tritium was developed. Purification of the labeled compound was performed on a TLC silica gel plate using a system of hexane-ethyl acetate 2:1. The molar radioactivity of [H-3]JH-III was 2.2 Ci mmol(-1). It was shown that tritium-labeled juvenile hormone can be used in the radiometric method for quantitative measurement of the activity of specific enzymes that hydrolyze juvenile hormone.
Cite:
Romanova I.V.
, Alekseev A.A.
, Richter V.A.
, Gruntenko N.E.
, Agafontsev A.M.
, Karpova E.K.
A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level
Russian Journal of Bioorganic Chemistry. 2017. V.43. N2. P.211-215. DOI: 10.1134/S1068162017020121 WOS Scopus РИНЦ OpenAlex
A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level
Russian Journal of Bioorganic Chemistry. 2017. V.43. N2. P.211-215. DOI: 10.1134/S1068162017020121 WOS Scopus РИНЦ OpenAlex
Dates:
| Published print: | Mar 1, 2017 |
| Published online: | Apr 19, 2017 |
Identifiers:
| Web of science: | WOS:000399743200015 |
| Scopus: | 2-s2.0-85017630693 |
| Elibrary: | 29487128 |
| OpenAlex: | W2607097220 |