Saccharides as Prospective Immobilizers of Nucleic Acids for Room-Temperature Structural EPR Studies

Saccharides as Prospective Immobilizers of Nucleic Acids for Room-Temperature Structural EPR Studies Full article

Journal

Journal of Physical Chemistry Letters
ISSN: 1948-7185

Output data

Year: 2016, Volume: 7, Number: 13, Pages: 2544-2548 Pages count : 5 DOI: 10.1021/acs.jpclett.6b01024

Authors

Kuzhelev Andrey A. ^1,4 , Shevelev Georgiy Yu ^2,4 , Krumkacheva Olesya A. ^3,4 , Tormyshev Victor M. ^1,4 , Pyshnyi Dmitrii V. ^2,4 , Fedin Matvey V. ^3,4 , Bagryanskaya Elena G. ^1,4

Affiliations

1	(Данные Web of science) RAS, NN Vorozhtsov Novosibirsk Inst Organ Chem, SB, Novosibirsk 630090, Russia
2	(Данные Web of science) RAS, Inst Chem Biol & Fundamental Med, SB, Novosibirsk 630090, Russia
3	(Данные Web of science) RAS, Int Tomog Ctr, SB, Novosibirsk 630090, Russia
4	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia

Pulsed dipolar electron paramagnetic resonance (EPR) spectroscopy is a powerful tool for structural studies of biomolecules and their complexes. This method, whose applicability has been recently extended to room temperatures, requires immobilization of the studied biosystem to prevent averaging of dipolar couplings; at the same time, the modification of native conformations by immobilization must be avoided. In this work, we provide first demonstration of room-temperature EPR distance measurements in nucleic acids using saccharides trehalose, sucrose, and glucose as immobilizing media. We propose an approach that keeps structural conformation and unity of immobilized double-stranded DNA. Remarkably, room-temperature electron spin dephasing time of triarylmethyl-labeled DNA in trehalose is noticeably longer compared to previously used immobilizers, thus providing a broader range of available distances. Therefore, saccharides, and especially trehalose, can be efficiently used as immobilizers of nucleic acids, mimicking native conditions and allowing wide range of structural EPR studies at room temperatures.

Kuzhelev A.A. , Shevelev G.Y. , Krumkacheva O.A. , Tormyshev V.M. , Pyshnyi D.V. , Fedin M.V. , Bagryanskaya E.G.
Saccharides as Prospective Immobilizers of Nucleic Acids for Room-Temperature Structural EPR Studies

Journal of Physical Chemistry Letters. 2016. V.7. N13. P.2544-2548. DOI: 10.1021/acs.jpclett.6b01024 WOS Scopus РИНЦ OpenAlex

Full text from publisher

Published online:	Jun 22, 2016
Published print:	Jul 7, 2016

Web of science:	WOS:000379457400043
Scopus:	2-s2.0-84978148913
Elibrary:	27016596
OpenAlex:	W2426989173

DB	Citing
Web of science	22
Scopus	22
Elibrary	24
OpenAlex	20