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Functional Electron Paramagnetic Resonance Imaging of Ischemic Rat Heart: Monitoring of Tissue Oxygenation and pH Научная публикация

Журнал Magnetic Resonance in Medicine
ISSN: 0740-3194
Вых. Данные Год: 2016, Том: 76, Номер: 1, Страницы: 350-358 Страниц : DOI: 10.1002/mrm.25867
Ключевые слова EPR imaging; EPR oximetry; spin probes; nitroxide radicals; myocardial ischemia; myocardial acidosis
Авторы Gorodetsky Artem A. 1,2 , Kirilyuk Igor A. 1,2 , Khramtsov Valery V. 3,4 , Komarov Denis A. 1,5
Организации
1 (Данные Web of science) Vorozhtsov Inst Organ Chem, Novosibirsk, Russia
2 (Данные Web of science) Novosibirsk State Univ, Novosibirsk, Russia
3 (Данные Web of science) Ohio State Univ, Heart & Lung Res Inst, Columbus, OH 43210 USA
4 (Данные Web of science) West Virginia Univ, Sch Med, Dept Biochem, Morgantown, WV USA
5 (Данные Web of science) Meshalkin State Res Inst Circulat Pathol, Novosibirsk, Russia

Реферат: Purpose: Electron paramagnetic resonance (EPR) imaging in the spectral-spatial domain with application of soluble paramagnetic probes provides an opportunity for spatially resolved functional measurements of living objects. The purpose of this study was to develop EPR methods for visualization of oxygenation and acidosis of ischemic myocardium. Methods: EPR oxygen measurements were performed using isotopically substituted 2 H, 15 N-dicarboxyproxyl. The radical has an EPR line width of 320 mG and oxygen-induced line broadening of 0.53 mG/mm Hg, providing oxygen sensitivity down to 5 mM. pH measurements were performed using previously developed pH-sensitive imidazoline nitroxide. The radical has an EPR spectrum with pH-dependable hyperfine splitting, pK = 6.6, providing pH sensitivity of approximately 0.05 U in the physiological range. Results: EPR imaging of isolated and perfused rat hearts was performed in the two-dimensional + spectral domain. The spatial resolution of the measurements was about 1.4 mm. Marked tissue hypoxia was observed in the ischemic area of the heart after occlusion of the left anterior descending coronary artery. Tissue oxygenation was partly restored upon reperfusion. EPR mapping of myocardial pH indicated acidosis of the ischemic area down to pH 6.7-6.8. Conclusion: This study demonstrates the capability of low-field EPR and the nitroxide spin probes for mapping of myocardial oxygenation and pH. The developed approaches might be used for noninvasive investigation of microenvironment on living objects. (C) 2015 Wiley Periodicals, Inc.
Библиографическая ссылка: Gorodetsky A.A. , Kirilyuk I.A. , Khramtsov V.V. , Komarov D.A.
Functional Electron Paramagnetic Resonance Imaging of Ischemic Rat Heart: Monitoring of Tissue Oxygenation and pH
Magnetic Resonance in Medicine. 2016. V.76. N1. P.350-358. DOI: 10.1002/mrm.25867 WOS Scopus РИНЦ OpenAlex
Файлы: Полный текст от издателя
Даты:
Опубликована online: 24 авг. 2015 г.
Опубликована в печати: 1 июл. 2016 г.
Идентификаторы БД:
Web of science: WOS:000384996900035
Scopus: 2-s2.0-84974827118
РИНЦ: 27127694
OpenAlex: W2143955238
Цитирование в БД:
БД Цитирований
Web of science 41
Scopus 45
РИНЦ 42
OpenAlex 44
Альметрики: