Реферат: first_pagesettingsOrder Article Reprints Open AccessArticle Calcification of Various Bioprosthetic Materials in Rats: Is It Really Different? by Irina Y. Zhuravleva 1,*,Elena V. Karpova 2ORCID,Anna A. Dokuchaeva 1,Anatoly T. Titov 3,Tatiana P. Timchenko 1 andMaria B. Vasilieva 1 1 E. Meshalkin National Medical Research Center of the RF Ministry of Health, 15 Rechkunovskaya St., 630055 Novosibirsk, Russia 2 N. Vorozhtsov Novosibirsk Institute of Organic Chemistry SB RAS, 9 Lavrentiev Avenue, 630090 Novosibirsk, Russia 3 V. Sobolev Institute of Geology and Mineralogy SB RAS, 3 Academician Koptyug Avenue, 630090 Novosibirsk, Russia * Author to whom correspondence should be addressed. Int. J. Mol. Sci. 2023, 24(8), 7274; https://doi.org/10.3390/ijms24087274 Original submission received: 17 March 2023 / Revised: 5 April 2023 / Accepted: 11 April 2023 / Published: 14 April 2023 (This article belongs to the Special Issue Biomaterials: From Design, Synthesis and Characterization to Application) Downloadkeyboard_arrow_down Browse Figures Review Reports Versions Notes Abstract The causes of heart valve bioprosthetic calcification are still not clear. In this paper, we compared the calcification in the porcine aorta (Ao) and the bovine jugular vein (Ve) walls, as well as the bovine pericardium (Pe). Biomaterials were crosslinked with glutaraldehyde (GA) and diepoxide (DE), after which they were implanted subcutaneously in young rats for 10, 20, and 30 days. Collagen, elastin, and fibrillin were visualized in non-implanted samples. Atomic absorption spectroscopy, histological methods, scanning electron microscopy, and Fourier-transform infrared spectroscopy were used to study the dynamics of calcification. By the 30th day, calcium accumulated most intensively in the collagen fibers of the GA-Pe. In elastin-rich materials, calcium deposits were associated with elastin fibers and localized differences in the walls of Ao and Ve. The DE-Pe did not calcify at all for 30 days. Alkaline phosphatase does not affect calcification since it was not found in the implant tissue. Fibrillin surrounds elastin fibers in the Ao and Ve, but its involvement in calcification is questionable. In the subcutaneous space of young rats, which are used to model the implants’ calcification, the content of phosphorus was five times higher than in aging animals. We hypothesize that the centers of calcium phosphate nucleation are the positively charged nitrogen of the pyridinium rings, which is the main one in fresh elastin and appears in collagen as a result of GA preservation. Nucleation can be significantly accelerated at high concentrations of phosphorus in biological fluids. The hypothesis needs further experimental confirmation.

Библиографическая ссылка: Zhuravleva I.Y. , Karpova E.V. , Dokuchaeva A.A. , Titov A.T. , Timchenko T.P. , Vasilieva M.B.
Calcification of Various Bioprosthetic Materials in Rats: Is It Really Different?
International Journal of Molecular Sciences. 2023. V.24. N8. P.7274. DOI: 10.3390/ijms24087274 WOS РИНЦ

Даты:

Принята к публикации:	11 апр. 2023 г.
Опубликована в печати:	14 апр. 2023 г.

Идентификаторы:

Web of science	WOS:000979229000001
РИНЦ	52301866
OpenAlex	W4365509899

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