A radical containing injectable in-situ-oleogel and emulgel for prolonged invivo oxygen measurements with CW EPR

A radical containing injectable in-situ-oleogel and emulgel for prolonged invivo oxygen measurements with CW EPR Научная публикация

Журнал

Free Radical Biology and Medicine
ISSN: 0891-5849

Вых. Данные

Год: 2019, Том: 130, Страницы: 120-127 Страниц : 8 DOI: 10.1016/j.freeradbiomed.2018.10.442

Авторы

Lampp Lisa ^1,5 , Rogozhnikova Olga Yu. ^2,3 , Trukhin Dmitry V. ^2,3 , Tormyshev Victor M. ^2,3 , Bowman Michael K. ⁴ , Devasahayam Nllathamby ⁵ , Krishna Murali C. ⁵ , Maeder Karsten ¹ , Imming Peter ¹

Организации

1	(Данные Web of science) Martin Luther Univ Halle Wittenberg, Inst Pharm, Wolfgang Langenbeck Str 4, D-06120 Halle, Saale, Germany
2	(Данные Web of science) RAS, SB, NN Vorozhtsov Novosibirsk Inst Organ Chem, 9 Lavrentjev Ave, Novosibirsk 630090, Russia
3	(Данные Web of science) Novosibirsk State Univ, Novosibirsk 630090, Russia
4	(Данные Web of science) Univ Alabama, Dept Chem & Biochem, Box 870336, Tuscaloosa, AL 35487 USA
5	(Данные Web of science) NCI, Radiat Biol Branch, Ctr Canc Res, NIH, Bldg 10, Bethesda, MD 20892 USA

Molecular oxygen, reactive oxygen species and free radicals derived from oxygen play important roles in a broad spectrum of physiological and pathological processes. The quantitative measurement of molecular oxygen in tissues by electron paramagnetic resonance (EPR) has great potential for understanding and diagnosing a number of diseases, and for developing and guiding therapies. This requires improvements in the free radical probe systems that sense and report molecular oxygen levels in vivo. We report on the encapsulation of existing free radical probes in lipophilic gel implants: an in-situ-oleogel and an emulgel, based only on well-known, safe excipients for the incorporation of lipophilic and hydrophilic radicals, respectively. The EPR signals of encapsulated radicals were not altered compared to dissolved radicals. The high solubility of oxygen in lipophilic solvents enhanced oxygen sensitivity. The gels extended the lifetime of the radicals in tissues from tens of minutes to many days, simplifying studies with extended series of measurements. The encapsulated radicals showed a good in vivo response to changes in oxygen supply and seem to circumvent concerns from toxicity of the radical probes. These gels simplify the development of new oxygen-sensitive free radical probes for EPR oximetry by making their in vivo stability, persistence and toxicity a function of the encapsulating gel and not a set of additional requirements for the free radical probe.

Lampp L. , Rogozhnikova O.Y. , Trukhin D.V. , Tormyshev V.M. , Bowman M.K. , Devasahayam N. , Krishna M.C. , Maeder K. , Imming P.
A radical containing injectable in-situ-oleogel and emulgel for prolonged invivo oxygen measurements with CW EPR

Free Radical Biology and Medicine. 2019. V.130. P.120-127. DOI: 10.1016/j.freeradbiomed.2018.10.442 WOS Scopus РИНЦ

Полный текст от издателя

Опубликована в печати:

1 янв. 2019 г.

Web of science	WOS:000455347000011
Scopus	2-s2.0-85056178856
РИНЦ	38628963
OpenAlex	W2898365956