Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy Научная публикация
Журнал |
Nucleic Acids Research
ISSN: 0305-1048 , E-ISSN: 1362-4962 |
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Вых. Данные | Год: 2018, Том: 46, Номер: 2, Страницы: 897-904 Страниц : 8 DOI: 10.1093/nar/gkx1136 | ||||||||
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Реферат:
The model mRNA (MR), 11-mer RNA containing two nitroxide spin labels at the 5'-and 3'-terminal nucleotides and prone to form a stable homodimer (MR) 2, was used for Electron Paramagnetic Resonance study of structural rearrangements in mRNA occurring upon its binding to human 80S ribosomes. The formation of two different types of ribosomal complexes with MR was observed. First, there were stable complexes where MR was fixed in the ribosomal mRNA-binding channel by the codon-anticodon interaction(s) with cognate tRNA(s). Second, we for the first time detected complexes assembled without tRNA due to the binding of MR most likely to an exposed peptide of ribosomal protein uS3 away from the mRNA channel. The analysis of interspin distances allowed the conclusion that 80S ribosomes facilitate dissociation of the duplex (MR) 2: the equilibrium between the duplex and the single-stranded MR shifts to MR due to its efficient binding with ribosomes. Furthermore, we observed a significant influence of tRNA bound at the ribosomal exit (E) and/ or aminoacyl (A) sites on the stability of ribosomal complexes. Our findings showed that a part of mRNA bound in the ribosome channel, which is not involved in codon-anticodon interactions, has more degrees of freedom than that interacting with tRNAs.
Библиографическая ссылка:
Malygin A.A.
, Graifer D.M.
, Meschaninova M.I.
, Venyaminova A.G.
, Timofeev I.O.
, Kuzhelev A.A.
, Krumkacheva O.A.
, Fedin M.V.
, Karpova G.G.
, Bagryanskaya E.G.
Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy
Nucleic Acids Research. 2018. V.46. N2. P.897-904. DOI: 10.1093/nar/gkx1136 WOS Scopus РИНЦ
Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy
Nucleic Acids Research. 2018. V.46. N2. P.897-904. DOI: 10.1093/nar/gkx1136 WOS Scopus РИНЦ
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Полный текст от издателя
Даты:
Опубликована online: | 16 нояб. 2017 г. |
Опубликована в печати: | 25 янв. 2018 г. |
Идентификаторы:
Web of science | WOS:000423812300037 |
Scopus | 2-s2.0-85044640684 |
РИНЦ | 35531377 |
OpenAlex | W2769846544 |