Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging

Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging Научная публикация

Журнал

Magnetic Resonance Materials in Physics, Biology and Medicine
ISSN: 0968-5243 , E-ISSN: 1352-8661

Вых. Данные

Год: 2017, Том: 30, Номер: 3, Страницы: 291-298 Страниц : 8 DOI: 10.1007/s10334-016-0606-8

Ключевые слова

Nitroxyl radical; Clonogenic assay; In vivo nitroxyl radical kinetics; In vivo EPR; T-2* mapping; Single-point imaging

Авторы

Kubota Harue ¹ , Komarov Denis A. ¹ , Yasui Hironobu ² , Matsumoto Shingo ¹ , Inanami Osamu ³ , Kirilyuk Igor A. ⁴ , Khramtsov Valery V. ⁵ , Hirata Hiroshi ¹

Организации

1	(Данные Web of science) Hokkaido Univ, Div Bioengn & Bioinformat, Grad Sch Informat Sci & Technol, Kita Ku, North 14,West 9, Sapporo, Hokkaido 0600814, Japan
2	(Данные Web of science) Hokkaido Univ, Cent Inst Isotope Sci, Kita Ku, North 15,West 7, Sapporo, Hokkaido 0600815, Japan
3	(Данные Web of science) Hokkaido Univ, Lab Radiat Biol, Grad Sch Vet Med, Kita Ku, North 18,West 9, Sapporo, Hokkaido 0600818, Japan
4	(Данные Web of science) NN Vorozhtsov Novosibirsk Inst Organ Chem, 9 Ac Lavrentieva Ave, Novosibirsk 630090, Russia
5	(Данные Web of science) West Virginia Univ, Dept Biochem, Robert C Byrd Hlth Sci Ctr, 1 Med Ctr Dr, Morgantown, WV 26506 USA

Objectives The aim of this study was to demonstrate the feasibility of in vivo three-dimensional (3D) relaxation time T-2* mapping of a dicarboxy-PROXYL radical using continuous-wave electron paramagnetic resonance (CW-EPR) imaging. Materials and methods Isotopically substituted dicarboxy-PROXYL radicals, 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-( 3,4-H-2(2))-(1-N-15)-1-oxyl (H-2, N-15-DCP) and 3,4-dicarboxy-2,2,5,5-tetra(H-2(3)) methylpyrrolidin-(3,4-H-2(2))1- oxyl (H-2-DCP), were used in the study. A clonogenic cell survival assay was performed with the H-2-DCP radical using squamous cell carcinoma (SCC VII) cells. The time course of EPR signal intensities of intravenously injected H-2, N-15-DCP and H-2-DCP radicals were determined in tumor-bearing hind legs of mice (C3H/HeJ, male, n = 5). CW-EPR-based single-point imaging (SPI) was performed for 3D T-2* mapping. Results H-2-DCP radical did not exhibit cytotoxicity at concentrations below 10 mM. The in vivo half-life of H-2, N-15-DCP in tumor tissues was 24.7 +/- 2.9 min (mean +/- standard deviation [SD], n = 5). The in vivo time course of the EPR signal intensity of the H-2, N-15-DCP radical showed a plateau of 10.2 +/- 1.2 min (mean +/- SD) where the EPR signal intensity remained at more than 90% of the maximum intensity. During the plateau, in vivo 3D T-2* maps with H-2, N-15-DCP were obtained from tumor-bearing hind legs, with a total acquisition time of 7.5 min. Conclusion EPR signals of H-2, N-15-DCP persisted long enough after bolus intravenous injection to conduct in vivo 3D T-2* mapping with CW-EPR-based SPI.

Kubota H. , Komarov D.A. , Yasui H. , Matsumoto S. , Inanami O. , Kirilyuk I.A. , Khramtsov V.V. , Hirata H.
Feasibility of in vivo three-dimensional T-2(*) mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging

Magnetic Resonance Materials in Physics, Biology and Medicine. 2017. V.30. N3. P.291-298. DOI: 10.1007/s10334-016-0606-8 WOS Scopus РИНЦ

Полный текст от издателя

Опубликована online:	6 янв. 2017 г.
Опубликована в печати:	1 июн. 2017 г.

Web of science	WOS:000401925200007
Scopus	2-s2.0-85008511984
РИНЦ	31042606
OpenAlex	W2571086721