The influence of zinc and silicate ions on biological properties of hydroxyapatite synthesized by a mechanochemical method

The influence of zinc and silicate ions on biological properties of hydroxyapatite synthesized by a mechanochemical method Научная публикация

Журнал

Ceramics International
ISSN: 0272-8842

Вых. Данные

Год: 2021, Том: 47, Номер: 7, Part A, Страницы: 9495-9503 Страниц : 9 DOI: 10.1016/j.ceramint.2020.12.083

Ключевые слова

Co-substituted hydroxyapatite; Implantation; In vivo; MG63; Osseointegration; Silicon; Zinc

Авторы

Khvostov Mikhail V. ^1,3 , Borisova Marina S. ³ , Bulina Natalia V. ^1,2 , Makarova Svetlana V. ¹ , Dumchenko Natalya B. ⁴ , Tolstikova Tatjana G. ³ , Lyakhov Nikolay Z. ¹

Организации

1	Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of Russian Academy of Sciences (SB RAS), Kutateladze Str. 18, Novosibirsk, 630128, Russian Federation
2	Novosibirsk State University, Pirogova Str. 1630090, Russian Federation
3	N. N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of Russian Academy of Sciences (SB RAS), Lavrentiev Avenue 9, Novosibirsk, 630090, Russian Federation
4	State Research Center of Virology and Biotechnology VECTOR, Federal Service for Surveillance in Consumer Rights Protection and Human Well-being, Koltsovo, Novosibirsk Oblast, 630559, Russian Federation

In this study, a mechanochemical method was applied to the synthesis of hydroxyapatite with double and triple substitution. Simultaneous replacement of phosphate ions by carbonate and silicate ions was implemented in the case of double substitution. The triple substitution involved additional replacement of a calcium ion by a zinc ion. The synthesized materials were characterized by X-ray diffraction and Fourier transform infrared spectroscopy. Samples of co-substituted hydroxyapatites in powder form were evaluated in vitro using human osteoblast cell line MG-63 and in vivo via implantation into a bone defect in the rat cranial vault. After 4 days of incubation in vitro, the hydroxyapatite containing zinc, silicon, and carbonate ions with composition Ca9.9Zn0.1(PO4)5.6(SiO4)0.2(CO3)0.2(OH)2 significantly increased cell proliferation in comparison with the other compositions. Four months after the implantation in vivo, this sample showed the greatest ability to integrate into rat skull bones, as evidenced by a morphological analysis of bone sections. © 2020 Elsevier Ltd and Techna Group S.r.l.

Khvostov M.V. , Borisova M.S. , Bulina N.V. , Makarova S.V. , Dumchenko N.B. , Tolstikova T.G. , Lyakhov N.Z.
The influence of zinc and silicate ions on biological properties of hydroxyapatite synthesized by a mechanochemical method
Ceramics International. 2021. V.47. N7, Part A. P.9495-9503. DOI: 10.1016/j.ceramint.2020.12.083 WOS Scopus

Опубликована в печати:

1 дек. 2020 г.

Web of science	WOS:000624008500002
Scopus	2-s2.0-85097660763
OpenAlex	W3111505076