Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies

Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies Научная публикация

Журнал

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN: 1570-0232 , E-ISSN: 1873-376X

Вых. Данные

Год: 2016, Том: 1036, Страницы: 136-141 Страниц : 6 DOI: 10.1016/j.jchromb.2016.10.009

Ключевые слова

Anti-influenza; Camphecene; Dried blood spots; LC-MS/MS

Авторы

Rogachev Artem D. ^1,2 , Yarovaya Olga I. ^1,2 , Ankov Sergey V. ¹ , Khvostov Mikhail V. ¹ , Tolstikova Tatyana G. ^1,2 , Pokrovsky Andrey G. ² , Salakhutdinov Nariman F. ^1,2

Организации

1	(Данные Web of science) Russian Acad Sci, Siberian Branch, NN Vorozhtsov Novosibirsk Inst Organ Chem, Lavrenteva Ave 9, Novosibirsk 630090, Russia
2	(Данные Web of science) Novosibirsk State Univ, Pirogova St 2, Novosibirsk 630090, Russia

A fast, selective and sensitive procedure for quantitation of the camphor-based anti-influenza agent camphecene in whole rat blood was developed and validated using dried blood spots and LC-MS/MS. The method was validated according to recommendations of the FDA and EMA in terms of selectivity, linearity, accuracy, precision, recovery, matrix factor, stability, and carry-over. Sample preparation included spotting 20 mu L of whole blood taken from the tail vein onto the paper, drying and extracting the analyte, followed by evaporation of the solvent and analysis of the residue. HPLC separations were run on a reversed-phase microcolumn; the time of analysis was less than 2 min. MS/MS detection was performed on a triple quadrupole mass-spectrometer using multiple reaction monitoring (MRM) mode. Transitions 196.4 -> 122.2/153.3 and 152.2 -> 93.1/107.2 were monitored for camphecene and 2-adamantylamine hydrochloride (internal standard), respectively. The intra-and inter-day precisions and accuracies, matrix factor, carry-over and recovery were within acceptable limits. Despite low extraction recovery (less than 2%), the sensitivity of the method was enough to detect the analyte in the concentration range 50-2500 ng/mL. The application of the method was shown in pharmacokinetic studies of camphecene in rats at a dose of 10 mg/kg. (C) 2016 Elsevier B.V. All rights reserved.

Rogachev A.D. , Yarovaya O.I. , Ankov S.V. , Khvostov M.V. , Tolstikova T.G. , Pokrovsky A.G. , Salakhutdinov N.F.
Development and validation of ultrafast LC-MS/MS method for quantification of anti-influenza agent camphecene in whole rat blood using dried blood spots and its application to pharmacokinetic studies
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2016. V.1036. P.136-141. DOI: 10.1016/j.jchromb.2016.10.009 WOS Scopus РИНЦ

Опубликована в печати:

1 нояб. 2016 г.

Web of science	WOS:000387193800015
Scopus	2-s2.0-84991584210
РИНЦ	27573001
OpenAlex	W2530386001