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Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus Full article

Journal Pharmaceuticals
ISSN: 1424-8247
Output data Year: 2022, Volume: 15, Number: 9, Article number : 1158, Pages count : 11 DOI: 10.3390/ph15091158
Tags antiviral activity; coumarin; terpene; LC-MS/MS; pharmacokinetics; RSV
Authors Nemolochnova Arina G. 1,2 , Rogachev Artem D. 1,2 , Сальникова Ольга П. 1,2 , Khomenko Tatyana M. 1 , Volcho Konstantin 1 , Yarovaya Olga I. 1,2 , Fatianova Alina V. 2 , Pokrovsky Andrey G. 2 , Salakhutdinov Nariman F. 1
Affiliations
1 N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences
2 Novosibirsk State University

Funding (1)

1 Российский Научный Фонд 21-13-00026

Abstract: The stability of a new coumarin derivative, agent K-142, bearing -pinene residue and possessing antiviral activity against respiratory syncytial virus (RSV) was studied in whole mice blood in vitro, and a method for its quantification in this matrix was developed and validated. The sample preparation method was precipitation of whole blood with a mixture of 0.2 M ZnSO4 with MeOH (2:8 v/v) containing 2-adamantylamine hydrochloride as an internal standard (IS). Analysis was carried out by HPLC-MS/MS using reversed phase chromatography and a triple quadrupole mass spectrometer 6500 QTRAP (SCIEX) in multiple reaction monitoring (MRM) mode. The transitions 351.2 ! 217.1 Da and 152.2 ! 93.1/107.2 Da were monitored for K-142 and the IS, respectively. The method was validated in terms of selectivity, calibration curve, LLOQ, accuracy and precision, stability, recovery and carry over. The developed method was used for a pharmacokinetics study of the compound after its oral administration to mice at a dose of 20 mg/kg.
Cite: Nemolochnova A.G. , Rogachev A.D. , Сальникова О.П. , Khomenko T.M. , Volcho K. , Yarovaya O.I. , Fatianova A.V. , Pokrovsky A.G. , Salakhutdinov N.F.
Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus
Pharmaceuticals. 2022. V.15. N9. 1158 :1-11. DOI: 10.3390/ph15091158 WOS Scopus РИНЦ
Dates:
Submitted: Jul 31, 2022
Published print: Sep 14, 2022
Published online: Sep 18, 2022
Identifiers:
Web of science WOS:000856673400001
Scopus 2-s2.0-85138667180
Elibrary 54068029
OpenAlex W4296350909
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