Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus

Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus Научная публикация

Журнал

Pharmaceuticals
ISSN: 1424-8247

Вых. Данные

Год: 2022, Том: 15, Номер: 9, Номер статьи : 1158, Страниц : 11 DOI: 10.3390/ph15091158

Ключевые слова

antiviral activity; coumarin; terpene; LC-MS/MS; pharmacokinetics; RSV

Авторы

Nemolochnova Arina G. ^1,2 , Rogachev Artem D. ^1,2 , Сальникова Ольга П. ^1,2 , Khomenko Tatyana M. ¹ , Volcho Konstantin ¹ , Yarovaya Olga I. ^1,2 , Fatianova Alina V. ² , Pokrovsky Andrey G. ² , Salakhutdinov Nariman F. ¹

Организации

1	Новосибирский институт органической химии им. Н.Н. Ворожцова СО РАН
2	Новосибирский национальный исследовательский государственный университет

Информация о финансировании (1)

Российский Научный Фонд

21-13-00026

The stability of a new coumarin derivative, agent K-142, bearing -pinene residue and possessing antiviral activity against respiratory syncytial virus (RSV) was studied in whole mice blood in vitro, and a method for its quantification in this matrix was developed and validated. The sample preparation method was precipitation of whole blood with a mixture of 0.2 M ZnSO4 with MeOH (2:8 v/v) containing 2-adamantylamine hydrochloride as an internal standard (IS). Analysis was carried out by HPLC-MS/MS using reversed phase chromatography and a triple quadrupole mass spectrometer 6500 QTRAP (SCIEX) in multiple reaction monitoring (MRM) mode. The transitions 351.2 ! 217.1 Da and 152.2 ! 93.1/107.2 Da were monitored for K-142 and the IS, respectively. The method was validated in terms of selectivity, calibration curve, LLOQ, accuracy and precision, stability, recovery and carry over. The developed method was used for a pharmacokinetics study of the compound after its oral administration to mice at a dose of 20 mg/kg.

Nemolochnova A.G. , Rogachev A.D. , Сальникова О.П. , Khomenko T.M. , Volcho K. , Yarovaya O.I. , Fatianova A.V. , Pokrovsky A.G. , Salakhutdinov N.F.
Stability Study, Quantification Method and Pharmacokinetics Investigation of a Coumarin–Monoterpene Conjugate Possessing Antiviral Properties against Respiratory Syncytial Virus
Pharmaceuticals. 2022. V.15. N9. 1158 :1-11. DOI: 10.3390/ph15091158 WOS Scopus РИНЦ

Поступила в редакцию:	31 июл. 2022 г.
Опубликована в печати:	14 сент. 2022 г.
Опубликована online:	18 сент. 2022 г.

Web of science	WOS:000856673400001
Scopus	2-s2.0-85138667180
РИНЦ	54068029
OpenAlex	W4296350909