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Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method Full article

Journal Chemosensors
ISSN: 2227-9040
Output data Year: 2023, Volume: 11, Number: 2, Pages: 135 Pages count : 1 DOI: 10.3390/chemosensors11020135
Tags SARS-CoV-2 virus; spike S; ELISA; RBD; dyes
Authors Mordvinova Ekaterina D. 1,2 , Nikitina Polina A. 3 , Yarovaya Olga I. 1 , Volosnikova Ekaterina A. 2 , Murashkin Denis E. 2 , Isaeva Anastasiya A. 2 , Koldaeva Tatiana Y. 3 , Perevalov Valery P. 3 , Salakhutdinov Nariman F. 1 , Shcherbakov Dmitriy N. 2
Affiliations
1 N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences
2 State Research Center of Virology and Biotechnology VECTOR
3 D. Mendeleyev University of Chemical Technology of Russia

Abstract: Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor.
Cite: Mordvinova E.D. , Nikitina P.A. , Yarovaya O.I. , Volosnikova E.A. , Murashkin D.E. , Isaeva A.A. , Koldaeva T.Y. , Perevalov V.P. , Salakhutdinov N.F. , Shcherbakov D.N.
Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method
Chemosensors. 2023. V.11. N2. P.135. DOI: 10.3390/chemosensors11020135 WOS Scopus РИНЦ
Dates:
Submitted: Dec 25, 2022
Accepted: Feb 10, 2023
Published print: Feb 13, 2023
Identifiers:
Web of science WOS:000938929200001
Scopus 2-s2.0-85148878043
Elibrary 60696500
OpenAlex W4320490888
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