Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method

Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method Научная публикация

Журнал

Chemosensors
ISSN: 2227-9040

Вых. Данные

Год: 2023, Том: 11, Номер: 2, Страницы: 135 Страниц : 1 DOI: 10.3390/chemosensors11020135

Ключевые слова

SARS-CoV-2 virus; spike S; ELISA; RBD; dyes

Авторы

Mordvinova Ekaterina D. ^1,2 , Nikitina Polina A. ³ , Yarovaya Olga I. ¹ , Volosnikova Ekaterina A. ² , Murashkin Denis E. ² , Isaeva Anastasiya A. ² , Koldaeva Tatiana Y. ³ , Perevalov Valery P. ³ , Salakhutdinov Nariman F. ¹ , Shcherbakov Dmitriy N. ²

Организации

1	Новосибирский институт органической химии им. Н.Н. Ворожцова СО РАН
2	Федеральное бюджетное учреждение науки Государственный научный центр вирусологии и биотехнологии Вектор
3	Российский химико-технологический университет имени Д. И. Менделеева

Angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, plays a crucial role in the pathogenesis of COVID-19. ACE2 targeting holds the promise for preventing and inhibiting SARS-CoV-2 infection. In this work, we describe the development and use of a test system based on competitive ELISA for the primary screening of potential antiviral compounds. We studied the activity of the library of dyes of different groups. Several dyes (ortho-cresolphthalein, eosin (free acid), eosin (Na salt)) that inhibited the interaction of ACE2 with the spike proteins of SARS-CoV-2 have been identified among the candidates. A potential antiviral drug, methylene blue, did not show activity in our study. We believe that our results can help in the further search for inhibitors of interaction between the coronavirus spike protein and ACE2 receptor.

Mordvinova E.D. , Nikitina P.A. , Yarovaya O.I. , Volosnikova E.A. , Murashkin D.E. , Isaeva A.A. , Koldaeva T.Y. , Perevalov V.P. , Salakhutdinov N.F. , Shcherbakov D.N.
Inhibitors of the RBD-ACE-2 Found among a Wide Range of Dyes by the Immunoassay Method
Chemosensors. 2023. V.11. N2. P.135. DOI: 10.3390/chemosensors11020135 WOS Scopus РИНЦ

Поступила в редакцию:	25 дек. 2022 г.
Принята к публикации:	10 февр. 2023 г.
Опубликована в печати:	13 февр. 2023 г.

Web of science	WOS:000938929200001
Scopus	2-s2.0-85148878043
РИНЦ	60696500
OpenAlex	W4320490888