A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level

A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level Full article

Journal

Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620

Output data

Year: 2017, Volume: 43, Number: 2, Pages: 211-215 Pages count : 5 DOI: 10.1134/S1068162017020121

Tags

juvenile hormone; tritium; JH esterase; Drosophila; Galleria

Authors

Romanova I.V. ¹ , Alekseev A.A. ² , Richter V.A. ¹ , Gruntenko N.E. ³ , Agafontsev A.M. ⁴ , Karpova E.K. ³

Affiliations

1	(Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
2	(Данные Web of science) Russian Acad Sci, Siberian Branch, Voevodsky Inst Chem Kinet & Combust, Novosibirsk 630090, Russia
3	(Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk 630090, Russia
4	(Данные Web of science) Russian Acad Sci, Vorozhtsov Novosibirsk Inst Organ Chem, Siberian Branch, Novosibirsk 630090, Russia

A method of insect juvenile hormone III (JH-III) labeling by thermally activated tritium was developed. Purification of the labeled compound was performed on a TLC silica gel plate using a system of hexane-ethyl acetate 2:1. The molar radioactivity of [H-3]JH-III was 2.2 Ci mmol(-1). It was shown that tritium-labeled juvenile hormone can be used in the radiometric method for quantitative measurement of the activity of specific enzymes that hydrolyze juvenile hormone.

Romanova I.V. , Alekseev A.A. , Richter V.A. , Gruntenko N.E. , Agafontsev A.M. , Karpova E.K.
A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level
Russian Journal of Bioorganic Chemistry. 2017. V.43. N2. P.211-215. DOI: 10.1134/S1068162017020121 WOS Scopus РИНЦ

Published print:	Mar 1, 2017
Published online:	Apr 19, 2017

Web of science	WOS:000399743200015
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