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A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level Научная публикация

Журнал Russian Journal of Bioorganic Chemistry
ISSN: 1068-1620
Вых. Данные Год: 2017, Том: 43, Номер: 2, Страницы: 211-215 Страниц : 5 DOI: 10.1134/S1068162017020121
Ключевые слова juvenile hormone; tritium; JH esterase; Drosophila; Galleria
Авторы Romanova I.V. 1 , Alekseev A.A. 2 , Richter V.A. 1 , Gruntenko N.E. 3 , Agafontsev A.M. 4 , Karpova E.K. 3
Организации
1 (Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia
2 (Данные Web of science) Russian Acad Sci, Siberian Branch, Voevodsky Inst Chem Kinet & Combust, Novosibirsk 630090, Russia
3 (Данные Web of science) Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Novosibirsk 630090, Russia
4 (Данные Web of science) Russian Acad Sci, Vorozhtsov Novosibirsk Inst Organ Chem, Siberian Branch, Novosibirsk 630090, Russia

Реферат: A method of insect juvenile hormone III (JH-III) labeling by thermally activated tritium was developed. Purification of the labeled compound was performed on a TLC silica gel plate using a system of hexane-ethyl acetate 2:1. The molar radioactivity of [H-3]JH-III was 2.2 Ci mmol(-1). It was shown that tritium-labeled juvenile hormone can be used in the radiometric method for quantitative measurement of the activity of specific enzymes that hydrolyze juvenile hormone.
Библиографическая ссылка: Romanova I.V. , Alekseev A.A. , Richter V.A. , Gruntenko N.E. , Agafontsev A.M. , Karpova E.K.
A Synthesis of Tritium-Labeled Juvenile Hormone and Radiometric Analysis of the Enzymatic Hydrolysis Level
Russian Journal of Bioorganic Chemistry. 2017. V.43. N2. P.211-215. DOI: 10.1134/S1068162017020121 WOS Scopus РИНЦ
Даты:
Опубликована в печати: 1 мар. 2017 г.
Опубликована online: 19 апр. 2017 г.
Идентификаторы:
Web of science WOS:000399743200015
Scopus 2-s2.0-85017630693
РИНЦ 29487128
OpenAlex W2607097220
Альметрики: