Calcium chelidonate: Semi‐synthesis, crystallography, and osteoinductive activity in vitro and in vivo

Calcium chelidonate: Semi‐synthesis, crystallography, and osteoinductive activity in vitro and in vivo Научная публикация

Журнал

Pharmaceuticals
ISSN: 1424-8247

Вых. Данные

Год: 2021, Том: 14, Номер: 6, Номер статьи : 579, Страниц : DOI: 10.3390/ph14060579

Ключевые слова

Calcium chelidonate; Osteogenic activity; Semi‐synthesis; X‐ray diffraction analysis

Авторы

Avdeeva E. ¹ , Porokhova E. ² , Khlusov I. ^2,3 , Rybalova T. ⁴ , Shults E. ⁵ , Litvinova L. ⁶ , Shupletsova V. ⁶ , Khaziakhmatova O. ⁶ , Sukhodolo I. ² , Belousov M. ¹

Организации

1	Department of Pharmaceutical Analysis, Siberian State Medical University, Tomsk, 634050, Russian Federation
2	Department of Morphology and General Pathology, Siberian State Medical University, Tomsk, 634050, Russian Federation
3	Research School of Chemistry & Applied Biomedical Sciences, Tomsk Polytechnic University, Tomsk, 634050, Russian Federation
4	Center of Spectral Investigations, Novosibirsk Institute of Organic Chemistry, Siberian Branch, Novosibirsk, 630090, Russian Federation
5	Laboratory of Medicinal Chemistry, Novosibirsk Institute of Organic Chemistry, Siberian Branch, Novosibirsk, 630090, Russian Federation
6	Center for Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad, 236041, Russian Federation

Calcium chelidonate [Ca(ChA)(H2O)3]n was obtained by semi‐synthesis using natural chelidonic acid. The structure of the molecular complex was determined by X‐ray diffraction analysis. The asymmetric unit of [Ca(ChA)(H2O)3]n includes chelidonic acid coordinated through three oxygen atoms, and three water ligands. The oxygen atoms of acid and oxygen atoms of water from each asymmetric unit are also coordinated to the calcium of another one, forming an infinite linear complex. Calcium geometry is close to the trigonal dodecahedron (D2d). The intra‐complex hydrogen bonds additionally stabilize the linear species, which are parallel to the axis. In turn the linear species are packed into the 3D structure through mutual intercomplex hydrogen bonds. The osteogenic activity of the semi‐synthetic CaChA was studied in vitro on 21‐day hAMMSC culture and in vivo in mice using ectopic (subcutaneous) implantation of CaP‐coated Ti plates saturated in vitro with syngeneic bone marrow. The enhanced extracellular matrix ECM mineralization in vitro and ectopic bone tissue formation in situ occurred while a water solution of calcium chelidonate at a dose of 10 mg/kg was used. The test substance promotes human adipose‐derived multipotent mesenchymal stromal/stem cells (hAMMSCs), as well as mouse MSCs to differentiate into osteoblasts in vitro and in vivo, respectively. Calcium chelidonate is non‐toxic and can stimulate osteoinductive processes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Avdeeva E. , Porokhova E. , Khlusov I. , Rybalova T. , Shults E. , Litvinova L. , Shupletsova V. , Khaziakhmatova O. , Sukhodolo I. , Belousov M.
Calcium chelidonate: Semi‐synthesis, crystallography, and osteoinductive activity in vitro and in vivo

Pharmaceuticals. 2021. V.14. N6. 579 . DOI: 10.3390/ph14060579 WOS Scopus РИНЦ

Полный текст от издателя

Опубликована online:

17 июн. 2021 г.

Web of science	WOS:000666200700001
Scopus	2-s2.0-85108870495
РИНЦ	46862305
OpenAlex	W3166492596